Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death
Identifieur interne : 004591 ( Main/Exploration ); précédent : 004590; suivant : 004592Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death
Auteurs : Zoltán N. Oltval [États-Unis] ; Curt L. Milliman [États-Unis] ; Stanley J. Korsmeyer [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1993.
English descriptors
- Teeft :
- 3fll, Acad, Acid homology, Acid sequence, Alternative transcripts, Amino, Amino acid, Amino acid residues, Amino acid sequence, Amino acids, Apoptosis, Apoptotic, Apoptotic cell death, Apoptotic death, Apoptotic stimulus, Baxa, Bcl2, Cdna, Cdna library, Cdna probe, Cdna sequence, Cell cdna library, Cell death, Cell line, Cell lines, Cell lysates, Cell survival, Cells transfected, Chromosomal breakpoint, Clone, Clones transfected, Cold spring harbor, Cold spring harbor laboratory press, Comparable levels, Control antibody, Control cells, Control vector, Cortical thymocytes, Cyanogen bromide, Cytosolic, Cytosolic form, Death repressor activity, Death stimulus, Deprivation, Edman degradation, Endogenous, Endogenous baxa, Endogenous murine, Exon, Exon boundaries, Exon probe, Experimental procedures, Expression vector, Extensive homology, Fetal calf serum, First cycle, Follicular lymphoma, Forms heterodimers, Forms homodimers, Genetic control, Genomic, Glacial acetic acid, High levels, Hockenbery, Homodimers, Homology, Human cells, Human hamster, Immunoprecipitated, Immunoprecipitates, Integral membrane protein, Korsmeyer, Last cycle, Lymphoma, Lysates, Lysis buffer, Many adult tissues, Molecular mass, Murine, Murine baxa, Murine cdna, Natl, Negative selection, Northern blot analysis, Overexpressed, Partial protection, Peptide, Peptide fragments, Percent viability, Phage clone, Polymerase cetus, Primary immunoprecipitates, Primer, Primer pool, Proc, Protein, Protein partner, Protein products, Protein sequence, Repressor, Rescue cells, Restriction analysis, Secondary immunoprecipitation, Sequencing, Signal anchor sequence, Speciesspecific biotinylated, Spleen virus, Stably transfected cells, Standard protocol, Substantial amounts, Transfected, Transgenic mice, Transmembrane domain, Triplicate cultures, Tsujimoto, Unpublished data, Viability assays, Washington university, Western blot analysis.
Abstract
Abstract: Bcl-2 protein is able to repress a number of apoptotic death programs. To investigate the mechanism of Bcl-2's effect, we examined whether Bcl-2 interacted with other proteins. We identified an associated 21 kd protein partner, Bax, that has extensive amino acid homology with Bcl-2, focused within highly conserved domains I and II. Bax is encoded by six exons and demonstrates a complex pattern of alternative RNA splicing that predicts a 21 kd membrane (α) and two forms of cytosolic protein (β and γ). Bax homodimerizes and forms heterodimers with Bcl-2 in vivo. Overexpressed Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line. Overexpressed Bax also counters the death repressor activity of Bcl-2. These data suggest a model in which the ratio of Bcl-2 to Bax determines survival or death following an apoptotic stimulus.
Url:
DOI: 10.1016/0092-8674(93)90509-O
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000E35
- to stream Istex, to step Curation: 000E35
- to stream Istex, to step Checkpoint: 001C02
- to stream Main, to step Merge: 004657
- to stream Main, to step Curation: 004591
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death</title>
<author><name sortKey="Oltval, Zoltan N" sort="Oltval, Zoltan N" uniqKey="Oltval Z" first="Zoltán N." last="Oltval">Zoltán N. Oltval</name>
</author>
<author><name sortKey="Milliman, Curt L" sort="Milliman, Curt L" uniqKey="Milliman C" first="Curt L." last="Milliman">Curt L. Milliman</name>
</author>
<author><name sortKey="Korsmeyer, Stanley J" sort="Korsmeyer, Stanley J" uniqKey="Korsmeyer S" first="Stanley J." last="Korsmeyer">Stanley J. Korsmeyer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:1B31A4D63603B8AFFF0532E3BE6C6E162A18B1AA</idno>
<date when="1993" year="1993">1993</date>
<idno type="doi">10.1016/0092-8674(93)90509-O</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-BCDP6WS3-9/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000E35</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000E35</idno>
<idno type="wicri:Area/Istex/Curation">000E35</idno>
<idno type="wicri:Area/Istex/Checkpoint">001C02</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001C02</idno>
<idno type="wicri:doubleKey">0092-8674:1993:Oltval Z:bcl:heterodimerizes:in</idno>
<idno type="wicri:Area/Main/Merge">004657</idno>
<idno type="wicri:Area/Main/Curation">004591</idno>
<idno type="wicri:Area/Main/Exploration">004591</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death</title>
<author><name sortKey="Oltval, Zoltan N" sort="Oltval, Zoltan N" uniqKey="Oltval Z" first="Zoltán N." last="Oltval">Zoltán N. Oltval</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Missouri (État)</region>
</placeName>
<wicri:cityArea>Howard Hughes Medical Institute Department of Medicine Washington University School of Medicine St. Louis</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Missouri (État)</region>
</placeName>
<wicri:cityArea>Howard Hughes Medical Institute Department of Pathology Washington University School of Medicine St. Louis</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Milliman, Curt L" sort="Milliman, Curt L" uniqKey="Milliman C" first="Curt L." last="Milliman">Curt L. Milliman</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Missouri (État)</region>
</placeName>
<wicri:cityArea>Howard Hughes Medical Institute Department of Medicine Washington University School of Medicine St. Louis</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Missouri (État)</region>
</placeName>
<wicri:cityArea>Howard Hughes Medical Institute Department of Pathology Washington University School of Medicine St. Louis</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Korsmeyer, Stanley J" sort="Korsmeyer, Stanley J" uniqKey="Korsmeyer S" first="Stanley J." last="Korsmeyer">Stanley J. Korsmeyer</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Missouri (État)</region>
</placeName>
<wicri:cityArea>Howard Hughes Medical Institute Department of Medicine Washington University School of Medicine St. Louis</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Missouri (État)</region>
</placeName>
<wicri:cityArea>Howard Hughes Medical Institute Department of Pathology Washington University School of Medicine St. Louis</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cell</title>
<title level="j" type="abbrev">CELL</title>
<idno type="ISSN">0092-8674</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1993">1993</date>
<biblScope unit="volume">74</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="609">609</biblScope>
<biblScope unit="page" to="619">619</biblScope>
</imprint>
<idno type="ISSN">0092-8674</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0092-8674</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>3fll</term>
<term>Acad</term>
<term>Acid homology</term>
<term>Acid sequence</term>
<term>Alternative transcripts</term>
<term>Amino</term>
<term>Amino acid</term>
<term>Amino acid residues</term>
<term>Amino acid sequence</term>
<term>Amino acids</term>
<term>Apoptosis</term>
<term>Apoptotic</term>
<term>Apoptotic cell death</term>
<term>Apoptotic death</term>
<term>Apoptotic stimulus</term>
<term>Baxa</term>
<term>Bcl2</term>
<term>Cdna</term>
<term>Cdna library</term>
<term>Cdna probe</term>
<term>Cdna sequence</term>
<term>Cell cdna library</term>
<term>Cell death</term>
<term>Cell line</term>
<term>Cell lines</term>
<term>Cell lysates</term>
<term>Cell survival</term>
<term>Cells transfected</term>
<term>Chromosomal breakpoint</term>
<term>Clone</term>
<term>Clones transfected</term>
<term>Cold spring harbor</term>
<term>Cold spring harbor laboratory press</term>
<term>Comparable levels</term>
<term>Control antibody</term>
<term>Control cells</term>
<term>Control vector</term>
<term>Cortical thymocytes</term>
<term>Cyanogen bromide</term>
<term>Cytosolic</term>
<term>Cytosolic form</term>
<term>Death repressor activity</term>
<term>Death stimulus</term>
<term>Deprivation</term>
<term>Edman degradation</term>
<term>Endogenous</term>
<term>Endogenous baxa</term>
<term>Endogenous murine</term>
<term>Exon</term>
<term>Exon boundaries</term>
<term>Exon probe</term>
<term>Experimental procedures</term>
<term>Expression vector</term>
<term>Extensive homology</term>
<term>Fetal calf serum</term>
<term>First cycle</term>
<term>Follicular lymphoma</term>
<term>Forms heterodimers</term>
<term>Forms homodimers</term>
<term>Genetic control</term>
<term>Genomic</term>
<term>Glacial acetic acid</term>
<term>High levels</term>
<term>Hockenbery</term>
<term>Homodimers</term>
<term>Homology</term>
<term>Human cells</term>
<term>Human hamster</term>
<term>Immunoprecipitated</term>
<term>Immunoprecipitates</term>
<term>Integral membrane protein</term>
<term>Korsmeyer</term>
<term>Last cycle</term>
<term>Lymphoma</term>
<term>Lysates</term>
<term>Lysis buffer</term>
<term>Many adult tissues</term>
<term>Molecular mass</term>
<term>Murine</term>
<term>Murine baxa</term>
<term>Murine cdna</term>
<term>Natl</term>
<term>Negative selection</term>
<term>Northern blot analysis</term>
<term>Overexpressed</term>
<term>Partial protection</term>
<term>Peptide</term>
<term>Peptide fragments</term>
<term>Percent viability</term>
<term>Phage clone</term>
<term>Polymerase cetus</term>
<term>Primary immunoprecipitates</term>
<term>Primer</term>
<term>Primer pool</term>
<term>Proc</term>
<term>Protein</term>
<term>Protein partner</term>
<term>Protein products</term>
<term>Protein sequence</term>
<term>Repressor</term>
<term>Rescue cells</term>
<term>Restriction analysis</term>
<term>Secondary immunoprecipitation</term>
<term>Sequencing</term>
<term>Signal anchor sequence</term>
<term>Speciesspecific biotinylated</term>
<term>Spleen virus</term>
<term>Stably transfected cells</term>
<term>Standard protocol</term>
<term>Substantial amounts</term>
<term>Transfected</term>
<term>Transgenic mice</term>
<term>Transmembrane domain</term>
<term>Triplicate cultures</term>
<term>Tsujimoto</term>
<term>Unpublished data</term>
<term>Viability assays</term>
<term>Washington university</term>
<term>Western blot analysis</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Bcl-2 protein is able to repress a number of apoptotic death programs. To investigate the mechanism of Bcl-2's effect, we examined whether Bcl-2 interacted with other proteins. We identified an associated 21 kd protein partner, Bax, that has extensive amino acid homology with Bcl-2, focused within highly conserved domains I and II. Bax is encoded by six exons and demonstrates a complex pattern of alternative RNA splicing that predicts a 21 kd membrane (α) and two forms of cytosolic protein (β and γ). Bax homodimerizes and forms heterodimers with Bcl-2 in vivo. Overexpressed Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line. Overexpressed Bax also counters the death repressor activity of Bcl-2. These data suggest a model in which the ratio of Bcl-2 to Bax determines survival or death following an apoptotic stimulus.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Missouri (État)</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Missouri (État)"><name sortKey="Oltval, Zoltan N" sort="Oltval, Zoltan N" uniqKey="Oltval Z" first="Zoltán N." last="Oltval">Zoltán N. Oltval</name>
</region>
<name sortKey="Korsmeyer, Stanley J" sort="Korsmeyer, Stanley J" uniqKey="Korsmeyer S" first="Stanley J." last="Korsmeyer">Stanley J. Korsmeyer</name>
<name sortKey="Korsmeyer, Stanley J" sort="Korsmeyer, Stanley J" uniqKey="Korsmeyer S" first="Stanley J." last="Korsmeyer">Stanley J. Korsmeyer</name>
<name sortKey="Milliman, Curt L" sort="Milliman, Curt L" uniqKey="Milliman C" first="Curt L." last="Milliman">Curt L. Milliman</name>
<name sortKey="Milliman, Curt L" sort="Milliman, Curt L" uniqKey="Milliman C" first="Curt L." last="Milliman">Curt L. Milliman</name>
<name sortKey="Oltval, Zoltan N" sort="Oltval, Zoltan N" uniqKey="Oltval Z" first="Zoltán N." last="Oltval">Zoltán N. Oltval</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004591 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004591 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:1B31A4D63603B8AFFF0532E3BE6C6E162A18B1AA |texte= Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death }}
This area was generated with Dilib version V0.6.33. |